PMID:8224242
| Citation |
Riedel, HD, Heinrich, J, Heisig, A, Choli, T and Schuster, H (1993) The antirepressor of phage P1. Isolation and interaction with the C1 repressor of P1 and P7. FEBS Lett. 334:165-9 |
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| Abstract |
Two antirepressor proteins, Ant1 and Ant2, of molecular weight 42 and 32 kDa, respectively, are encoded by P1 as a single open reading frame, with the smaller protein initiating at an in-frame start codon. Another open reading frame, icd, 5' upstream of and overlapping ant1 is required for ant1 expression. Using appropriate ant gene-carrying plasmids we have overproduced and purified Ant1/2 in the form of a protein complex and Ant2 as a single protein. Sequence analysis confirmed the N-terminal amino acids predicted from the DNA sequence of ant1/ant2, except that the N-terminal methionine is missing in the Ant2 protein. Under appropriate conditions the C1 repressors of phages P1 and P7 specifically co-precipitate with the Ant1/2 complex but not with Ant2 protein alone. The results suggest that the antirepressor may exert its C1-inactivating function by a direct protein-protein interaction. |
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| Keywords |
Amino Acid Sequence; Bacteriophage P1/genetics; Bacteriophage P1/metabolism; Base Sequence; Coliphages/genetics; Coliphages/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Escherichia coli/metabolism; Molecular Sequence Data; Molecular Weight; Operon; Plasmids; Repressor Proteins/biosynthesis; Repressor Proteins/isolation & purification; Repressor Proteins/metabolism; Restriction Mapping; Viral Proteins/biosynthesis; Viral Proteins/isolation & purification; Viral Proteins/metabolism |
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