PMID:8223625

Citation	Voelkner, P, Puppe, W and Altendorf, K (1993) Characterization of the KdpD protein, the sensor kinase of the K(+)-translocating Kdp system of Escherichia coli. Eur. J. Biochem. 217:1019-26
Abstract	KdpD and KdpE, proteins that control expression of the kdpFABC operon, are members of the class of sensor kinase/response regulator proteins. Using polyclonal antibodies raised against the KdpD protein, we have been able to identify and to localize the chromosome-encoded KdpD protein in the cytoplasmic membrane of Escherichia coli. Furthermore, it has been possible to detect differences in the expression of the KdpD protein according to the K+ concentration in the growth medium. The phosphorylation capacity of the plasmid-encoded KdpD protein and the phospho-transfer to KdpE was investigated. We found that both reactions were strictly dependent on the ionic conditions of the assay medium. Based on optimized conditions, we were able to detect phosphorylation of the chromosome-encoded KdpD protein. Furthermore, replacement of the conserved histidine (His673), the predicted phosphorylation site in KdpD, by glutamine revealed that phosphorylation of KdpD was no longer possible.
Links	PubMed
Keywords	Antibodies; Bacterial Proteins/genetics; Bacterial Proteins/immunology; Bacterial Proteins/metabolism; Biological Transport; Chromosomes, Bacterial; Escherichia coli/enzymology; Escherichia coli Proteins; Operon; Phosphorylation; Phosphotransferases/metabolism; Plasmids; Potassium/metabolism; Protein Kinases/genetics; Protein Kinases/immunology; Protein Kinases/metabolism
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.