PMID:8195102
| Citation |
Scott, JH and Nealson, KH (1994) A biochemical study of the intermediary carbon metabolism of Shewanella putrefaciens. J. Bacteriol. 176:3408-11 |
|---|---|
| Abstract |
Cell extracts were used to determine the enzymes involved in the intermediary carbon metabolism of several strains of Shewanella putrefaciens. Enzymes of the Entner-Doudoroff pathway (6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase) were detected, but those of the Embden-Meyerhof-Parnas pathway were not. While several tricarboxylic acid cycle enzymes were present under both aerobic and anaerobic conditions, two key enzymes (2-oxoglutarate dehydrogenase and pyruvate dehydrogenase) were greatly diminished under anaerobic conditions. Extracts of cell grown anaerobically on formate as the sole source of carbon and energy were positive for hydroxypyruvate reductase, the key enzyme of the serine pathway in other methylotrophs, while no hexulose synthase activity was seen. |
| Links | |
| Keywords |
Aerobiosis; Alcohol Oxidoreductases/analysis; Aldehyde-Lyases/analysis; Anaerobiosis; Bacteria/enzymology; Bacteria/metabolism; Carbon/metabolism; Citric Acid Cycle; Formates/metabolism; Glucose/metabolism; Hydro-Lyases/analysis; Hydroxypyruvate Reductase; Models, Biological; Serine/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.