PMID:8188615
| Citation |
Matsushima, Y, Kim, DW, Yoshimura, T, Kuramitsu, S, Kagamiyama, H, Esaki, N and Soda, K (1994) Replacement of active-site lysine-239 of thermostable aspartate aminotransferase by S-(2-aminoethyl)cysteine: properties of the mutant enzyme. J. Biochem. 115:108-12 |
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| Abstract |
The active-site lysine residue of thermostable aspartate aminotransferase, Lys-239, to which the cofactor, pyridoxal 5'-phosphate (PLP), is bound, has been converted to Cys by site-directed mutagenesis. The thiol group of Cys-239 was chemically aminoethylated with ethylenimine. Amino acid analysis of the modified enzyme showed that it contained about 1 mol of S-(2-aminoethyl)cysteine (SAEC) per mol subunit. The activity of the mutant enzyme (K239SAEC) was about 14% of that of the wild-type enzyme. No significant difference in thermostability was found between the wild-type and K239SAEC enzymes. The UV-visible spectrum of K239SAEC showed a peak (lambda max 380 nm), due to absorption by the cofactor, at a 20 nm longer wavelength than that of the wild-type enzyme. The circular dichroism band due to the bound cofactor of K239SAEC also shifted toward a 20 nm longer wavelength. We determined kinetic parameters (rate constants, kmax, and dissociation constants, Kd, for the substrates) for each half transamination catalyzed by the wild-type and K239SAEC mutant enzymes by the stopped-flow method. The kmax values for the mutant enzyme reactions were 2.6-24 times lower than those for the wild-type enzyme ones. The two enzymes showed similar Kd values for the same substrates except glutamate; the mutant enzyme showed higher affinity for glutamate than the wild-type enzyme. |
| Links | |
| Keywords |
Aspartate Aminotransferases/chemistry; Aspartate Aminotransferases/metabolism; Binding Sites; Cysteine/analogs & derivatives; Cysteine/chemistry; Cysteine/metabolism; Enzyme Stability; Escherichia coli/genetics; Hydrogen-Ion Concentration; Kinetics; Lysine/chemistry; Lysine/metabolism; Models, Chemical; Mutagenesis, Site-Directed; Mutation; Pyridoxal Phosphate/metabolism; Temperature; Thermodynamics |
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