PMID:8157609
| Citation |
Zhao, N, Oh, W, Trybul, D, Thrasher, KS, Kingsbury, TJ and Larson, TJ (1994) Characterization of the interaction of the glp repressor of Escherichia coli K-12 with single and tandem glp operator variants. J. Bacteriol. 176:2393-7 |
|---|---|
| Abstract |
The glp operons of Escherichia coli are negatively controlled by the glp repressor. Comparison of the repressor-binding affinities for consensus and altered consensus operators in vivo showed that all base substitutions at positions 3, 4, 5, and 8 from the center of the palindromic operator caused a striking decrease in repressor binding. Substitutions at other positions had a severe to no effect on repressor binding, depending on the base substitution. The results obtained indicate that the repressor binds with highest affinity to operators with the half-site WATKYTCGWW, where W is A or T, K is G or T, and Y is C or T. Strong cooperative binding of the repressor to tandem operators was demonstrated in vivo. Cooperativity was maximal when two 20-bp operators were directly repeated or when 2 bp separated the two operators. Cooperativity decreased with the deletion of 2 bp or the addition of 4 bp between the individual operators. Cooperativity was eliminated with a 6-bp insertion between the operators. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Gene Expression; Genes, Bacterial/genetics; Genes, Bacterial/physiology; Molecular Sequence Data; Operator Regions, Genetic/physiology; Operon/physiology; Regulon/physiology; Repetitive Sequences, Nucleic Acid/genetics; Repetitive Sequences, Nucleic Acid/physiology; Repressor Proteins/genetics; Repressor Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.