PMID:8144477

Citation	Lee, CY, O'Kane, DJ and Meighen, EA (1994) Riboflavin synthesis genes are linked with the lux operon of Photobacterium phosphoreum. J. Bacteriol. 176:2100-4
Abstract	Four genes immediately downstream of luxG in the Photobacterium phosphoreum lux operon (ribEBHA) have been sequenced and shown to be involved in riboflavin synthesis. Sequence analyses and complementation of Escherichia coli riboflavin auxotrophs showed that the gene products of ribB and ribA are 3,4-dihydroxy-2-butanone 4-phosphate (DHBP) synthetase and GTP cyclohydrolase II, respectively. By expression of P. phosphoreum ribE in E. coli using the bacteriophage T7 promoter-RNA polymerase system, ribE was shown to code for riboflavin synthetase, which catalyzes the conversion of lumazine to riboflavin. Increased thermal stability of RibE on expression with RibH indicated that ribH coded for lumazine synthetase. The organization of the rib genes in P. phosphoreum is quite distinct, with ribB and ribA being linked but separated by ribH, whereas in E. coli, they are unlinked and in Bacillus subtilis, RibB and RibA functions are coded by a single gene.
Links	PubMed PMC205317
Keywords	Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Escherichia coli/genetics; Genes, Bacterial/genetics; Genetic Complementation Test; Genetic Linkage; Molecular Sequence Data; Operon/genetics; Oxidoreductases; Photobacterium/genetics; Riboflavin/biosynthesis; Sequence Analysis, DNA
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