PMID:8135540
| Citation |
Schrum, LW and Hassan, HM (1994) The effects of fur on the transcriptional and post-transcriptional regulation of MnSOD gene (sodA) in Escherichia coli. Arch. Biochem. Biophys. 309:288-92 |
|---|---|
| Abstract |
Earlier studies have shown that the Fur (ferric uptake regulation) protein acts as an anaerobic/aerobic repressor of MnSOD (sodA) expression. We found that the aerobic expression of sodA::lacZ fusion in a Fur- background to be threefold higher than in a Fur+ background. This effect of fur mutation was not seen in a strain harboring the sodA+ gene instead of the sodA::lacZ fusion. However, we observed a proportionate increase in the concentrations of sodA::lacZ and sodA+ mRNAs in response to a mutation in the fur gene. These data suggest that the formation of active MnSOD is dependent on a functional fur gene. Indeed, we found that in a fur mutant iron was incorporated into SodA in place of manganese, thus creating inactive and/or partially active forms of the enzyme (i.e., Fe2SodA and/or Mn,FeSodA, respectively), resulting in little or no increase in total MnSOD activity. Thus, Fur plays the role of a repressor at the transcriptional level, but it also plays an indirect role at the post-transcriptional level where it affects the maturation of SodA into a fully active enzyme, Mn2SodA (MnSOD). |
| Links |
PubMed Online version:10.1006/abbi.1994.1115 |
| Keywords |
Aerobiosis; Bacterial Proteins/pharmacology; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial/drug effects; Iron/metabolism; Manganese; Mutation; Plasmids; RNA, Messenger/metabolism; Recombinant Fusion Proteins/metabolism; Repressor Proteins/pharmacology; Superoxide Dismutase/genetics; Superoxide Dismutase/metabolism; Transcription, Genetic/drug effects; beta-Galactosidase/genetics |
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