PMID:8132474
| Citation |
Choi, KY and Zalkin, H (1994) Role of the purine repressor hinge sequence in repressor function. J. Bacteriol. 176:1767-72 |
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| Abstract |
A protease-hypersensitive hinge sequence in Escherichia coli purine repressor (PurR) connects an N-terminal DNA-binding domain with a contiguous corepressor-binding domain. Binding of one molecule of dimeric repressor to operator DNA protects the hinge against proteolytic cleavage. Mutations in the hinge region impair repressor function in vivo. Several nonfunctional hinge mutants were defective in low-affinity binding to operator DNA in the absence of corepressor as well as in high-affinity corepressor-dependent binding to operator DNA, although binding of corepressor was similar to binding of the wild-type repressor. These results establish a role for the hinge region in operator binding and lead to a proposal for two routes to form the holoPurR-operator complex. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/physiology; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Escherichia coli Proteins; Models, Genetic; Molecular Sequence Data; Mutation; Repressor Proteins/genetics; Repressor Proteins/metabolism; Repressor Proteins/physiology |
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