PMID:8125312

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Citation

Schlaman, HR, Risseeuw, E, Franke-van Dijk, ME and Hooykaas, PJ (1994) Nucleotide sequence corrections of the uidA open reading frame encoding beta-glucuronidase. Gene 138:259-60

Abstract

Part of the open reading frame of uidA, encoding beta-glucuronidase, was sequenced and two differences were found with the previously reported nucleotide sequence [Jefferson et al., Proc. Natl. Acad. Sci. USA 83 (1986) 8447-8451]. One is a silent mutation, the other results in the Glu279-->Gln substitution.

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Keywords

Amino Acid Sequence; Artifacts; Base Sequence; Cloning, Molecular/methods; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Glucuronidase/genetics; Molecular Sequence Data; Open Reading Frames; Point Mutation; Polymerase Chain Reaction/methods

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