PMID:8107146
| Citation |
Celia, H, Hoermann, L, Schultz, P, Lebeau, L, Mallouh, V, Wigley, DB, Wang, JC, Mioskowski, C and Oudet, P (1994) Three-dimensional model of Escherichia coli gyrase B subunit crystallized in two-dimensions on novobiocin-linked phospholipid films. J. Mol. Biol. 236:618-28 |
|---|---|
| Abstract |
Two-dimensional crystals of the Escherichia coli DNA gyrase B subunit were obtained upon specific interactions with novobiocin linked phospholipid films. A three-dimensional surface model of the protein was generated by analysing images of tilted negatively stained crystals. The structure showed, at 2.5 to 3.0 nm resolution, two elongated arms organised as a V-shaped protein: the bottom of the V contains the novobiocin binding site, and the extremities of the arms mediate protein-protein interactions between the two monomers in the unit cell. Image analysis of frozen hydrated two-dimensional crystals resulted in a 1.0 nm resolution projection map that shows structural elements not revealed with negative staining. Electron microscopic structural data were compared with the crystallographic structure of the 43 kDa N-terminal fragment of the B subunit complexed with a non hydrolysable ATP analogue. |
| Links |
PubMed Online version:10.1006/jmbi.1994.1171 |
| Keywords |
Binding Sites; Crystallography, X-Ray; DNA Gyrase; DNA Topoisomerases, Type II/chemistry; DNA Topoisomerases, Type II/ultrastructure; Escherichia coli/chemistry; Escherichia coli/enzymology; Escherichia coli/ultrastructure; Image Processing, Computer-Assisted; Microscopy, Electron; Models, Molecular; Novobiocin/chemistry; Phospholipids/chemistry; Water/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.