PMID:8022285
| Citation |
Craig, JE, Zhang, Y and Gallagher, MP (1994) Cloning of the nupC gene of Escherichia coli encoding a nucleoside transport system, and identification of an adjacent insertion element, IS 186. Mol. Microbiol. 11:1159-68 |
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| Abstract |
Escherichia coli is known to contain more than one active transport system for nucleoside uptake. In the present study we report the sequence of a gene encoding a second nucleoside transport system, nupC (in addition to nupG). An open reading frame (ORF) of 1200 bp was identified that codes for a hydrophobic polypeptide of 43,560 Da and an NupC fusion protein was shown to be membrane associated. The native NupC protein is also identified, following over-expression. NupC exhibits short regions of homology to several membrane-associated proteins, including LacY and Cyd. Analysis of the nupC promoter region revealed the presence of at least two putative CRP-binding sites, centred at -40bp and -89bp, which probably flank a CytR-binding site. In addition, an adjacent IS 186 element was identified and found to reside within a putative terminator structure, downstream from the nupC ORF. This arrangement is shown to reflect the previously established gene order on the E. coli chromosome. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Base Sequence; Biological Transport/genetics; Carrier Proteins/biosynthesis; Carrier Proteins/genetics; Cloning, Molecular; Cyclic AMP Receptor Protein/metabolism; DNA Transposable Elements/genetics; DNA, Bacterial/metabolism; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial/genetics; Membrane Transport Proteins; Molecular Sequence Data; Nucleosides/metabolism; Promoter Regions, Genetic/genetics; Protein Binding; Recombinant Proteins/biosynthesis; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Terminator Regions, Genetic |
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