PMID:8021226
| Citation |
Menon, NK, Chatelus, CY, Dervartanian, M, Wendt, JC, Shanmugam, KT, Peck, HD Jr and Przybyla, AE (1994) Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2. J. Bacteriol. 176:4416-23 |
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| Abstract |
The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA Mutational Analysis; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Hydrogenase/chemistry; Hydrogenase/genetics; Hydrogenase/metabolism; Molecular Sequence Data; Operon; Phenotype; Sequence Analysis, DNA |
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