PMID:8011018
| Citation |
Lu, M, Campbell, JL, Boye, E and Kleckner, N (1994) SeqA: a negative modulator of replication initiation in E. coli. Cell 77:413-26 |
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| Abstract |
In E. coli, replication initiates at a genetically unique origin, oriC. Rapidly growing cells contain multiple oriC copies. Initiation occurs synchronously, once and only once per cell cycle at all origins present. Secondary initiations are prevented by a sequestration process that acts uniquely on newly replicated origins, which are marked because they are hemimethylated at GATC sites. We report the identification of a gene required for sequestration and demonstrate that this gene, seqA, also serves as a negative modulator of the primary initiation process. All previously identified in vivo initiation factors play positive roles. Thus, precise control of replication initiation may involve a balance between positive and negative elements. We suggest that SeqA might be a cooperativity factor, acting to make the replication initiation process dependent upon cooperative interactions among components. |
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| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Base Sequence; Chromosomes, Bacterial; Cloning, Molecular; DNA Replication/genetics; DNA, Bacterial/analysis; DNA, Bacterial/biosynthesis; DNA, Bacterial/metabolism; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/genetics; Genes, Bacterial/genetics; Genetic Complementation Test; Methylation; Methyltransferases/genetics; Models, Genetic; Molecular Sequence Data; Phenotype; Restriction Mapping; SOS Response (Genetics)/genetics; Sequence Analysis, DNA; Sequence Deletion/genetics; Site-Specific DNA-Methyltransferase (Adenine-Specific); Suppression, Genetic/genetics; Transcription Factors |
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