PMID:8001824
| Citation |
Tang, H, Severinov, K, Goldfarb, A, Fenyo, D, Chait, B and Ebright, RH (1994) Location, structure, and function of the target of a transcriptional activator protein. Genes Dev. 8:3058-67 |
|---|---|
| Abstract |
We have isolated and characterized single-amino-acid substitution mutants of RNA polymerase alpha subunit defective in CAP-dependent transcription at the lac promoter but not defective in CAP-independent transcription. Our results establish that (1) amino acids 258-265 of alpha constitute an "activation target" essential for CAP-dependent transcription at the lac promoter but not essential for CAP-independent transcription, (2) amino acid 261 is the most critical amino acid of the activation target, (3) amino acid 261 is distinct from the determinants for alpha-DNA interaction, and (4) the activation target may fold as a surface amphipathic alpha-helix. We propose a model for transcriptional activation at the lac promoter that integrates these and other recent results regarding transcriptional activation and RNA polymerase structure and function. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cyclic AMP Receptor Protein/metabolism; DNA Primers; DNA, Bacterial/metabolism; DNA-Directed RNA Polymerases/biosynthesis; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Macromolecular Substances; Molecular Sequence Data; Mutagenesis; Oligodeoxyribonucleotides; Plasmids; Point Mutation; Promoter Regions, Genetic; Transcription, Genetic |
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