PMID:7994604
| Citation |
Ferat, JL, Le Gouar, M and Michel, F (1994) Multiple group II self-splicing introns in mobile DNA from Escherichia coli. C. R. Acad. Sci. III, Sci. Vie 317:141-8 |
|---|---|
| Abstract |
By PCR (polymerase chain reaction) amplification and cloning, we have identified four group II self-splicing introns encoding proteins related to reverse transcriptases in natural Escherichia coli isolates belonging to the ECOR collection. One intron, IntD, interrupts a DNA sequence virtually identical to that of the previously described IS3411 Insertion Sequence. A second intron, IntC, is located within an open reading frame that is closely related to a reading frame in the T-DNA of Agrobacterium tumefaciens. Finally, introns IntA and IntB are inserted at two distinct sites in one of the Rhs elements of E. coli. A comparison of their open reading frames shows that the two Rhs introns are more closely related to each other than to any other known group II intron: this suggests that transposition of group II introns may occur preferentially in cis, along the same piece of DNA. |
| Links | |
| Keywords |
DNA Transposable Elements/drug effects; DNA Transposable Elements/genetics; Escherichia coli/genetics; Introns/genetics; Molecular Sequence Data; Open Reading Frames/genetics; Phylogeny; Polymerase Chain Reaction; RNA, Catalytic/pharmacology |
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