PMID:7961841
| Citation |
Welter, R, Gu, LQ, Yu, L, Yu, CA, Rumbley, J and Gennis, RB (1994) Identification of the ubiquinol-binding site in the cytochrome bo3-ubiquinol oxidase of Escherichia coli. J. Biol. Chem. 269:28834-8 |
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| Abstract |
The cytochrome bo3-ubiquinol oxidase, one of two ubiquinol oxidases in Escherichia coli, is a member of the heme-copper oxidase superfamily. The enzyme contains four protein subunits (I-IV) with apparent molecular masses of 58, 33, 22, and 17 kDa, respectively. Cytochrome bo3 catalyzes the 2-electron oxidation of ubiquinol and the reduction of molecular oxygen to water. Although the primary structures of all four subunits have been determined, the ubiquinol-binding site has not been investigated. The photoreactive radiolabeled azidoubiquinone derivative 3-[3H]azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinone (azido-Q), which has been widely used in locating the ubiquinone-binding sites of other enzymes, was used to identify the subunit(s) involved in the binding of quinol to cytochrome bo3. When reduced by dithioerythritol, the azido-Q derivative functioned as a substrate with partial effectiveness, suggesting that azido-Q interacts with a legitimate quinol-binding site. When cytochrome bo3 was incubated with an 8-fold molar excess of azido-Q, illumination by UV light for 10 min resulted in a 50% loss of activity. The uptake of radiolabeled azido-Q by the oxidase complex upon illumination correlated with the photoinactivation. In the presence of the competitive inhibitor 2-heptyl-4-hydroxyquinoline or ubiquinol, the rate of azido-Q uptake and the loss of enzyme activity upon illumination decreased. Analysis of the distribution of radioactivity among the subunits after separation by SDS-polyacrylamide gel electrophoresis showed that subunit II was heavily labeled by azido-Q, but that the other subunits were not. This suggests that the ubiquinol-binding site of the cytochrome bo3 complex is located at least partially on subunit II. |
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| Keywords |
Affinity Labels; Azides/metabolism; Binding Sites; Cytochromes/chemistry; Cytochromes/metabolism; Electron Transport Complex IV/antagonists & inhibitors; Electron Transport Complex IV/chemistry; Electron Transport Complex IV/metabolism; Escherichia coli/enzymology; Escherichia coli Proteins; Quinones/chemistry; Quinones/pharmacology; Radioisotopes; Substrate Specificity; Ubiquinone/analogs & derivatives; Ubiquinone/metabolism |
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