PMID:7961507
| Citation |
Liu, X and Matsumura, P (1994) The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons. J. Bacteriol. 176:7345-51 |
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| Abstract |
The Escherichia coli flhD operon encodes two genes, flhD and flhC. Both gene products were overproduced and purified. The purified proteins formed a complex consisting of two FlhD and two FlhC molecules. Mobility shift assays showed that the FlhD/FlhC complex had a DNA-binding activity and bound to the upstream regions of fliA, flhB, and fliL operons (class II), which are under direct control of the flhD operon. DNase I footprinting analyses of FlhD/FlhC binding to the three class II promoter regions revealed protection of a 48-bp region of the fliA operon between positions -41 to -88, a 50-bp region of the flhB operon between positions -28 to -77, and a 48-bp region of the fliL operon between positions -29 to -76. In vitro transcription experiments demonstrated that the FlhD/FlhC complex is a transcriptional activator required for the transcription of the three class II operons examined in vitro. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Base Sequence; DNA, Bacterial/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli Proteins; Flagella/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Operon/genetics; Promoter Regions, Genetic/genetics; Protein Binding; Recombinant Proteins/metabolism; Sigma Factor/genetics; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription, Genetic; Transcriptional Activation |
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