PMID:7961413
| Citation |
Lamblin, AF and Fuchs, JA (1994) Functional analysis of the Escherichia coli K-12 cyn operon transcriptional regulation. J. Bacteriol. 176:6613-22 |
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| Abstract |
The cynTSX operon enables Escherichia coli K-12 to degrade and use cyanate as a sole nitrogen source. The promoter of this operon is positively regulated by cyanate and the CynR protein. CynR, a member of the LysR family of regulatory proteins, binds specifically to a 136-bp DNA fragment containing both the cynR and the cynTSX promoters. In this study, we report the results of DNase I digestion studies showing that CynR protects a 60-bp region on the cynR coding strand and a 56-bp sequence on the cynTSX coding strand. CynR binding was not affected by cyanate or its structural homolog azide, a gratuitous inducer of the operon. However, CynR-induced bending of two different DNA fragments was detected. The amount of bending was decreased by cyanate. |
| Links | |
| Keywords |
Azides/metabolism; Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Carbon-Nitrogen Lyases; Carbonic Anhydrases/genetics; Cyanates/metabolism; DNA-Binding Proteins; Enzyme Induction; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Lyases/genetics; Molecular Sequence Data; Nucleic Acid Conformation; Operon/genetics; Promoter Regions, Genetic/genetics; Protein Binding; Trans-Activators; Transcription Factors/metabolism; Transcription, Genetic |
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