PMID:7934869

Citation	Lin, YS, Kieser, HM, Hopwood, DA and Chen, CW (1993) The chromosomal DNA of Streptomyces lividans 66 is linear. Mol. Microbiol. 10:923-33
Abstract	Two copies of a DNA sequence similar or identical to one end of the linear plasmid SLP2 were found on the Streptomyces lividans chromosome. Restriction mapping showed that these sequences represented free ends. Electrophoretic retardation and glass-binding studies indicated that the telomeres carry covalently bound proteins. Moreover, the chromosome migrated as an 8 Mb linear DNA in pulsed-field gel electrophoresis. A similar finding with the chromosomes of six other Streptomyces species suggested that a linear chromosome may be characteristic of the genus. The S. lividans chromosome can be circularized by joining the two ends by artificial targeted recombination or by spontaneous deletions spanning both telomeres. Thus the chromosome appears to be able to exist, in viable bacteria, as a linear or a circular molecule.
Links	PubMed
Keywords	Bacterial Proteins/chemistry; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial/chemistry; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; DNA, Circular/chemistry; Genome, Bacterial; Molecular Sequence Data; Multigene Family; Nucleic Acid Conformation; Plasmids/genetics; Streptomyces/chemistry; Streptomyces/genetics
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