PMID:7934862
| Citation |
Sinai, AP and Bavoil, PM (1993) Hyper-invasive mutants define a novel Pho-regulated invasion pathway in Escherichia coli. Mol. Microbiol. 10:1125-37 |
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| Abstract |
We have isolated two transposon insertion mutations of the pst-phoU operon which result in the constitutive expression of the phoA gene product, alkaline phosphatase. The two mutations also render Escherichia coli invasive towards cultured HEp-2 cells and define a novel Pho-regulated invasion pathway. The presence of the large 'invasion' plasmid derived from an entero-invasive E. coli (EIEC) clinical isolate in these mutants leads to enhanced invasiveness toward cultured HEp-2 cells, a phenomenon referred to as the 'hyper-invasive' phenotype. Transduction of a pst-phoU insertion mutation into clinical isolates of EIEC and Shigella flexneri results in constitutive PhoA expression and coupled hyper-invasiveness in the former but not the latter. We speculate that the Pho-regulated invasion pathway described here, while silent in bacteria grown in standard laboratory rich media, may become functional in the host when invasive bacteria encounter nutrient starvation and/or other related stress conditions. |
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| Keywords |
Alkaline Phosphatase/genetics; Antigens, Bacterial/genetics; Base Sequence; Cell Line; Chromosome Mapping; DNA Transposable Elements; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/pathogenicity; Gene Expression Regulation, Bacterial; Genes, Bacterial; Humans; Molecular Sequence Data; Mutation; Plasmids/genetics; Shigella flexneri/genetics; Shigella flexneri/pathogenicity; Virulence/genetics |
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