PMID:7934849
| Citation |
Gidrol, X and Farr, S (1993) Interaction of a redox-sensitive DNA-binding factor with the 5'-flanking region of the micF gene in Escherichia coli. Mol. Microbiol. 10:877-84 |
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| Abstract |
The product of the micF gene is an endogenous antisense RNA which down-regulates the expression of a major outer membrane protein, OmpF, in E. coli. We report here that two DNA-binding factors compete for the same site in the promoter region of the micF gene: RSBF, a high-affinity redox-sensitive DNA-binding factor that responds to an active oxygen species other than hydrogen peroxide or superoxide anions; and HRBF a heat-resistant DNA-binding factor. Both RSBF and HRBF bind to the same DNA sequence, 5'-TTAAAATCAATAACTTATTCTTAA3-', located upstream of the transcription start site of the micF gene. We present evidence that RSBF could be the controlling factor of a novel regulon involved in the response to oxidative stress in E. coli. |
| Links | |
| Keywords |
Bacterial Outer Membrane Proteins/genetics; Base Sequence; Binding Sites/genetics; Binding, Competitive; DNA Primers/genetics; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial/drug effects; Genes, Bacterial; Molecular Sequence Data; Oxidation-Reduction; Oxidative Stress; Paraquat/pharmacology; RNA, Antisense/genetics; RNA, Antisense/metabolism |
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