PMID:7928977
| Citation |
Zhao, G and Winkler, ME (1994) An Escherichia coli K-12 tktA tktB mutant deficient in transketolase activity requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth. J. Bacteriol. 176:6134-8 |
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| Abstract |
We show that a tktA tktB double mutant, which is devoid of the two known transketolase isoenzymes of Escherichia coli K-12, requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth. This pyridoxine requirement can also be satisfied by 4-hydroxy-L-threonine or glycolaldehyde. These results provide direct evidence that D-erythrose-4-phosphate is a precursor of the pyridine ring of pyridoxine. In addition, they show that the two major E. coli transketolase isoenzymes are not required for the biosynthesis of D-1-deoxyxylulose, which is thought to be another precursor of pyridoxine. |
| Links | |
| Keywords |
Amino Acids/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial/genetics; Isoenzymes/genetics; Isoenzymes/metabolism; Mutation; Pyridoxine/metabolism; Transketolase/genetics; Transketolase/metabolism; Vitamins/metabolism |
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