PMID:7905263

Citation	Kalapos, MP, Cao, GJ, Kushner, SR and Sarkar, N (1994) Identification of a second poly(A) polymerase in Escherichia coli. Biochem. Biophys. Res. Commun. 198:459-65
Abstract	A second poly(A) polymerase (PAP II) has been identified in Escherichia coli using a strain carrying a deletion of pcnB (the structural gene for PAP I; Cao and Sarkar, 1992b) and pnp-7 (a null mutation in the structural gene for polynucleotide phosphorylase). While PAP I has a M(r) of 53,000, PAP II is a smaller protein with a native M(r)-35,000. PAP II differs from PAP I in preferring poly(A) over tRNA primers and being more thermolabile. The presence of multiple poly(A) polymerases in E. coli raises interesting questions regarding the role of polyadenylation in mRNA synthesis and decay.
Links	PubMed Online version:10.1006/bbrc.1994.1067
Keywords	Bacterial Proteins/genetics; Escherichia coli/enzymology; Escherichia coli Proteins; Gene Deletion; Poly A/analysis; Polynucleotide Adenylyltransferase/isolation & purification; Polynucleotide Adenylyltransferase/metabolism; Polyribonucleotide Nucleotidyltransferase/deficiency; Polyribonucleotide Nucleotidyltransferase/genetics; RNA, Messenger/analysis; RNA, Messenger/metabolism
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