PMID:7891559
| Citation |
Py, B, Causton, H, Mudd, EA and Higgins, CF (1994) A protein complex mediating mRNA degradation in Escherichia coli. Mol. Microbiol. 14:717-29 |
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| Abstract |
mRNA degradation in Escherichia coli is mediated by a combination of exo- and endoribonucleases. We present evidence for a multiprotein complex which includes at least two enzymes that play important roles in mRNA degradation: the exoribonuclease polynucleotide phosphorylase (PNPase) and the endoribonuclease RNase E. An activity which impedes the processive activity of PNPase at stem-loop structures also appears to be associated with the complex. This complex is estimated to have a molecular mass of about 500 kDa and includes several additional polypeptides whose functions are unknown. The identification of a complex which includes several activities associated with mRNA degradation has implications for the mechanisms and co-ordinated control of mRNA degradation. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Base Sequence; Cytoplasm/metabolism; DNA, Bacterial/genetics; Endoribonucleases/genetics; Endoribonucleases/isolation & purification; Endoribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Microscopy, Immunoelectron; Molecular Sequence Data; Multienzyme Complexes/genetics; Multienzyme Complexes/isolation & purification; Multienzyme Complexes/metabolism; Polyribonucleotide Nucleotidyltransferase/genetics; Polyribonucleotide Nucleotidyltransferase/isolation & purification; Polyribonucleotide Nucleotidyltransferase/metabolism; RNA Processing, Post-Transcriptional; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/isolation & purification; RNA-Binding Proteins/metabolism |
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