PMID:7875569
| Citation |
Vasudevan, SG, Tang, P, Dixon, NE and Poole, RK (1995) Distribution of the flavohaemoglobin, HMP, between periplasm and cytoplasm in Escherichia coli. FEMS Microbiol. Lett. 125:219-24 |
|---|---|
| Abstract |
The subcellular distribution of the soluble flavohaemoglobin (HMP) of Escherichia coli has been determined. Cells over-expressing HMP from the cloned hmp gene on a multicopy plasmid were fractionated by osmotic shock and lysozyme treatment. Spectral analysis of subcellular fractions showed the CO-binding haemoprotein to be cytoplasmic. However, Western blotting using antibody raised to purified HMP revealed approximately 30% of the protein to be periplasmic in the over-expressing strain. Western analysis also revealed substantial levels of periplasmic HMP in a strain expressing only chromosomally encoded protein but none in an hmp mutant. The results are discussed in relation to protein function and the similar distribution reported for Vitreoscilla globin. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/analysis; Bacterial Proteins/biosynthesis; Bacterial Proteins/chemistry; Carbon Monoxide/metabolism; Cytoplasm/chemistry; Dihydropteridine Reductase; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Flavoproteins/analysis; Genes, Bacterial; Globins/chemistry; Hemeproteins/analysis; Hemeproteins/biosynthesis; Hemeproteins/chemistry; Molecular Sequence Data; NADH, NADPH Oxidoreductases; Sequence Homology, Amino Acid; Subcellular Fractions/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
