PMID:7875565

Citation	Cavard, D (1995) Role of DegP protease on levels of various forms of colicin A lysis protein. FEMS Microbiol. Lett. 125:173-8
Abstract	The total amount of the colicin A lysis protein produced by cells grown in rich medium was analysed by immunoblotting. The intermediate forms of synthesis of this small lipoprotein were present in the cells at any time of induction, confirming that processing and maturation of colicin A lysis protein are slow and incomplete processes. The level of these various forms varied according to the time of induction, the growth conditions, the producing strain and the plasmid carrying the cal gene. It depended mainly on the presence in the producing strain of a degP gene which encodes the DegP protease. According to growth conditions, the DegP protease hydrolysed either a part or the total amount of the acylated precursor form. In some cases, a protease(s) other than DegP seemed to act on either form(s) of the colicin A lysis protein.
Links	PubMed
Keywords	Bacterial Proteins/biosynthesis; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes, Fungal; Heat-Shock Proteins; Immunoblotting; Kinetics; Lipoproteins; Magnesium/pharmacology; Mutation; Periplasmic Proteins; Plasmids; Serine Endopeptidases/metabolism; Species Specificity
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.