PMID:7867952
| Citation |
Sundström, L, Jansson, C, Bremer, K, Heikkilä, E, Olsson-Liljequist, B and Sköld, O (1995) A new dhfrVIII trimethoprim-resistance gene, flanked by IS26, whose product is remote from other dihydrofolate reductases in parsimony analysis. Gene 154:7-14 |
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| Abstract |
A new plasmid-borne gene, dhfrVIII, encoding high-level trimethoprim resistance (TpR) was found in an intestinal Escherichia coli. It seems to be a widely occurring mediator of TpR. Among 973 examined TpR E. coli, the new resistance gene was found in 13 (1.3%) isolates from Sweden, Finland and Nigeria. The new gene was sequenced and found to code for a dihydrofolate reductase (DHFR) of 169 amino acids (M(r) 19005). The dhfrVIII gene on the studied plasmid pLMO226 was observed to be flanked by IS26 elements. The dhfrVIII gene and a 3' unidentified open reading frame (ORF) seem to be borne on a compound transposon with IS26 at its ends, since the configuration of two IS26 flanking dhfrVIII and the unidentified ORF was conserved among the isolates that were probe-positive for the gene. Phylogeny parsimony analysis showed the dhfrVIII-encoded enzyme to be only remotely related to other known plasmid-mediated, drug-resistant DHFR. Only a few of the latter form well-supported monophyletic groups. |
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| Keywords |
Amino Acid Sequence; Animal Population Groups/genetics; Animals; Bacteria/enzymology; Bacteria/genetics; Bacterial Proteins/genetics; Bacteriophages/enzymology; Bacteriophages/genetics; Base Sequence; DNA Transposable Elements; Escherichia coli/genetics; Molecular Sequence Data; Phylogeny; Plants/genetics; Sequence Alignment; Sequence Homology, Amino Acid; Tetrahydrofolate Dehydrogenase/genetics; Trimethoprim Resistance/genetics; Yeasts/enzymology; Yeasts/genetics |
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