PMID:7860602
| Citation |
Liu, J and Magasanik, B (1995) Activation of the dephosphorylation of nitrogen regulator I-phosphate of Escherichia coli. J. Bacteriol. 177:926-31 |
|---|---|
| Abstract |
The transcription of sigma 54 RNA polymerase-dependent nitrogen-regulated genes is activated by nitrogen regulator I (NRI)-phosphate. The kinase NRII is responsible for the phosphorylation of NRI. It has been shown that NRII also has the ability to dephosphorylate NRI-phosphate but only when PII is present at a concentration greatly in excess of that of NRII. We have now shown that glutamate enables PII to stimulate the dephosphorylation of NRI-phosphate when present in equimolar concentration with NRII. This effect of glutamate appears to be a backup control that becomes effective when the normal regulation of PII activity is disabled. |
| Links | |
| Keywords |
Bacterial Proteins/drug effects; Bacterial Proteins/metabolism; Cobalt/pharmacology; DNA-Binding Proteins/metabolism; DNA-Directed RNA Polymerases/metabolism; Dose-Response Relationship, Drug; Escherichia coli/enzymology; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Glutamate-Ammonia Ligase/biosynthesis; Glutamic Acid/pharmacology; Ketoglutaric Acids/pharmacology; PII Nitrogen Regulatory Proteins; Phosphorylation; Protein Kinases/metabolism; RNA Polymerase Sigma 54; Sigma Factor/metabolism; Trans-Activators; Transcription Factors |
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