PMID:7860583
| Citation |
Rech, S, Deppenmeier, U and Gunsalus, RP (1995) Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availability. J. Bacteriol. 177:1023-9 |
|---|---|
| Abstract |
The mod (chlD) locus at 17 min on the Escherichia coli chromosome encodes a high-affinity molybdate uptake system. To further investigate the structure and regulation of these genes, the DNA region upstream of the previously identified modBC (chlJD) genes was cloned and sequenced. A single open reading frame, designated modA, was identified and appears to encode a periplasmic binding protein for the molybdate uptake system. To determine how the mod genes are regulated in response to molybdate, nitrate, and oxygen, we constructed a series of mod-lacZ operon fusions to the upstream region and introduced them in single copy onto the E. coli chromosome. Whereas molybdate limitation resulted in elevated mod-lacZ expression, neither oxygen nor nitrate had any significant effect on gene expression. A regulatory motif, CATAA, located at the modA promoter was identified and shown to be required for molybdate-dependent control of the modABCD operon. Mutations within this sequence resulted in nearly complete derepression of gene expression and suggest that transcription of the operon is mediated by a molybdenum-responsive regulatory protein. |
| Links | |
| Keywords |
Alleles; Bacterial Proteins/genetics; Base Sequence; Biological Transport; Coenzymes; DNA-Binding Proteins; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Membrane Proteins/genetics; Metalloproteins/metabolism; Molecular Sequence Data; Molybdenum/metabolism; Mutation; Nitrates/pharmacology; Operon/genetics; Oxygen/pharmacology; Phosphoproteins/genetics; Promoter Regions, Genetic/genetics; Protein Kinases; Pteridines/metabolism; Recombinant Fusion Proteins/biosynthesis; Repressor Proteins/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.