PMID:783033
| Citation |
Chen, R (1976) The sequence determination of a protein in a micro scale: the sequence analysis of ribosomal protein L34 of Escherichia coli. Hoppe-Seyler's Z. Physiol. Chem. 357:873-86 |
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| Abstract |
The complete amino acid sequence of ribosomal protein L34 has been established by improved micro techniques with 3 mg of the lyophilized protein. The protein was digested with trypsin, thermolysin and chymotrypsin and the resulting peptides were isolated from fingerprints performed on cellulose thin-layer plates. The amino acid sequences of the peptides were determined by the combined micro dansyl-Edman technique using 5 - 10 nmol per sample. Aspartic acid and glutamic acid were distinguished from their amides by use of the color reaction of ninhydrin with the respective amino acid phenylthiohydantoins. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins; Chromatography, Thin Layer; Chymotrypsin; Dansyl Compounds; Escherichia coli/analysis; Hydrolysis; Microchemistry; Peptide Fragments/analysis; Ribosomal Proteins; Trypsin |
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