PMID:7815304

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Citation

de Rekarte, UD, Cortés, M, Porco, A, Niño, G and Istúriz, T (1994) Mutations affecting gluconate catabolism in Escherichia coli. Genetic mapping of loci for the low affinity transport and the thermoresistant gluconokinase. J. Basic Microbiol. 34:363-70

Abstract

The isolation and properties of strains of Escherichia coli carrying mutations affecting either the low affinity transport for gluconate (gene gntU) or the thermoresistant gluconokinase (gene gntK) are described. A lesion of each type was genetically characterized by transduction experiments. Both mutations mapped in the asd region, and the order was malA-glpD-asd-gntU-gntK, with the last two markers at about min 75.78 and 75.86 on the map, respectively. Mutations altering specifically gntU have not been previously reported.

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Keywords

Biological Transport; Chromosome Mapping; Enzyme Stability; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial/genetics; Gluconates/metabolism; Hot Temperature; Mutation; Phosphotransferases (Alcohol Group Acceptor)/genetics; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Transduction, Genetic

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