PMID:7813890
| Citation |
Beck, R, Crooke, H, Jarsch, M, Cole, J and Burtscher, H (1994) Mutation in dipZ leads to reduced production of active human placental alkaline phosphatase in Escherichia coli. FEMS Microbiol. Lett. 124:209-14 |
|---|---|
| Abstract |
We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the mutant but could be fully recovered by expression of dipZ subcloned in a vector with low copy number. Overexpression of E. coli alkaline phosphatase was unaffected in the mutant with or without dipZ co-expression. |
| Links | |
| Keywords |
Alkaline Phosphatase/biosynthesis; Alkaline Phosphatase/genetics; Escherichia coli/genetics; Female; Humans; Mutation; Oxidoreductases/biosynthesis; Placenta/enzymology; Plasmids; Pregnancy; Recombinant Proteins/biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.