PMID:7811102
| Citation |
Aono, R, Negishi, T and Nakajima, H (1994) Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli. Appl. Environ. Microbiol. 60:4624-6 |
|---|---|
| Abstract |
A variety of genes are involved in determining the level of organic solvent tolerance of Escherichia coli K-12. Gene ostA is one of the genes contributing to the level of organic solvent tolerance. This gene was cloned from an n-hexane-tolerant strain of E. coli, JA300. A JA300-based n-hexane-sensitive strain, OST4251, was converted to the n-hexane-tolerant phenotype by transformation with DNA containing the ostA gene derived from JA300. Thus, the cloned ostA gene complemented the n-hexane-sensitive phenotype of OST4251. |
| Links | |
| Keywords |
Cloning, Molecular; Drug Resistance, Microbial/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial/physiology; Genetic Complementation Test; Hexanes/pharmacology; Open Reading Frames/physiology; Restriction Mapping; Solvents |
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