PMID:7805834
| Citation |
Quail, MA, Dempsey, CE and Guest, JR (1994) Identification of a fatty acyl responsive regulator (FarR) in Escherichia coli. FEBS Lett. 356:183-7 |
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| Abstract |
FarR (formerly P30) has been identified as a fatty acid and fatty acyl-CoA responsive DNA-binding protein. It is encoded by the farR gene (g30) in the citric acid cycle gene cluster of E. coli (gltA-sdhCDAB-sucABCD-farR). The amplified FarR protein specifically bound to the farR promoter (PfarR) and exhibited weak binding to the citrate synthase and lipoamide dehydrogenase promoters. Binding at PfarR was abolished by long-chain fatty acids and their CoA thioesters. In DNaseI footprints, FarR binding at PfarR protected two sites, each characterised by two related 10-bp direct repeats. It is suggested that FarR autoregulates farR expression and may modulate citric acid cycle expression in response to long-chain fatty acids. |
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| Keywords |
Acyl Coenzyme A/pharmacology; Base Sequence; Chromatography, Affinity; Chromatography, Ion Exchange; Citrate (si)-Synthase/genetics; Citric Acid Cycle/genetics; DNA Primers; DNA-Binding Proteins/genetics; DNA-Binding Proteins/isolation & purification; DNA-Binding Proteins/metabolism; Dihydrolipoamide Dehydrogenase/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Fatty Acids, Nonesterified/pharmacology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Multigene Family; Promoter Regions, Genetic; Transcription Factors |
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