PMID:7801635
| Citation |
Wang, A, Chen, X, Sun, T and Pan, R (1994) [The cloning of threonine operon in vivo and mutagenesis in vitro in E. coli]. Wei Sheng Wu Xue Bao 34:266-70 |
|---|---|
| Abstract |
Report here was the selection of the E. coli strain producing 1.2% threonine. The wild type threonine operon was cloned in vivo and subcloned in vitro. The subclone was mutagenizied in vitro and a clone pTHR12-9-1 producing threonine was obtained. Also the effect of pTHR12-9-1 on threonine yield of hosts producing different amount of threonine was studied. We found that pTHR12-9-1 could promote the production of threonine in lower yield hosts, but it reduced the production of threonine in higher yield hosts. The phenomenon was discussed. |
| Links | |
| Keywords |
Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Mutagenesis; Operon; Threonine/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.