PMID:7789526

Citation	Dijkstra, AJ, Hermann, F and Keck, W (1995) Cloning and controlled overexpression of the gene encoding the 35 kDa soluble lytic transglycosylase from Escherichia coli. FEBS Lett. 366:115-8
Abstract	The lytic transglycosylases of Escherichia coli are involved in peptidoglycan metabolism and resemble the lysozymes not only in activity, but in the case of the 70 kDa soluble lytic transglycosylase (Slt70), also structurally. Here we report the cloning of the gene that encodes the 35 kDa soluble lytic transglycosylase (Slt35) of E. coli. Based on the sequence of the full-length gene, Slt35 is very likely to be a proteolytically truncated form of a slightly larger protein. The homology between Slt35 and Slt70, albeit poor, indicates that the active site architecture of both proteins may be alike. Using the T-7 promoter system, Slt35 was overproduced in large quantities and purified to homogeneity for crystallographic purposes.
Links	PubMed
Keywords	Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Base Sequence; Binding Sites; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Glycosyltransferases/biosynthesis; Glycosyltransferases/genetics; Molecular Sequence Data; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/genetics; Sequence Alignment
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.