PMID:7786035
| Citation |
Benov, L, Chang, LY, Day, B and Fridovich, I (1995) Copper, zinc superoxide dismutase in Escherichia coli: periplasmic localization. Arch. Biochem. Biophys. 319:508-11 |
|---|---|
| Abstract |
Cu,ZnSOD purified from Escherichia coli has been used to raise antibodies in rabbits. The resultant antiserum was found to recognize a single band on Western blots of SDS-polyacrylamide gel electropherograms, and that single band coincided with the position of the Cu,ZnSOD. Ultrathin sections of fixed E. coli were treated with the antibody followed by protein A bearing 10-nm gold particles. Electron microscopy revealed that Cu,ZnSOD was largely localized in the periplasm in polar bays. |
| Links |
PubMed Online version:10.1006/abbi.1995.1324 |
| Keywords |
Animals; Antibodies; Copper/chemistry; Escherichia coli/enzymology; Escherichia coli/ultrastructure; Immunohistochemistry; Microscopy, Electron; Rabbits; Superoxide Dismutase/analysis; Superoxide Dismutase/chemistry; Superoxide Dismutase/isolation & purification; Zinc/chemistry |
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