PMID:778334
| Citation |
Kornberg, HL and Riordan, C (1976) Uptake of galactose into Escherichia coli by facilitated diffusion. J. Gen. Microbiol. 94:75-89 |
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| Abstract |
Strains of Escherichia coli devoid of systems for the active transport of galactose (galP mgl) still grow on galactose but at rates that are a function of the galactose concentration of the medium: half-maximal growth rates require more than 2 mM-galactose to be present. Evidence is presented that galactose is taken up by such strains by facilitated diffusion on a carrier specified by the umg gene (or by a gene highly co-transducible with it) which is thus a part of, or closely associated with, an enzyme II for glucose of the phosphoenolpyruvate-phosphotransferase system. However, the entry of galactose does not require phosphotransferase activity, and the sugar taken up appears in the cells as free galactose. |
| Links | |
| Keywords |
Diffusion; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Galactose/metabolism; Genes; Glucose/pharmacology; Methylgalactosides/metabolism; Mutation; Phosphotransferases/metabolism |
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