PMID:7748923

Citation	McGovern, V, Higgins, NP, Chiz, RS and Jaworski, A (1994) H-NS over-expression induces an artificial stationary phase by silencing global transcription. Biochimie 76:1019-29
Abstract	Bacteria organize their chromosomes in a complex interwound supercoiled structure called the nucleoid through the action of topoisomerases and a set of small (10-20 kDa) proteins. The two most abundant nucleoid-associated proteins are HU and H-NS. H-NS increases in abundance during stationary phase. Over-expression of HU is well tolerated and compatible with transcription and cell growth. Increasing the concentration of H-NS leads to a rapid silencing of global transcription and produces a growth-arrested state reminiscent of stationary phase. H-NS over-expression also induces a substantial loss of supercoiling in plasmid DNA during the time that transcription is arrested. Comparing the effects of over-expression of these two proteins gives some insight into the differential roles of these proteins in the activity of the chromosome. These observations are interpreted in a model of nucleoid organization.
Links	PubMed
Keywords	Bacterial Outer Membrane Proteins/biosynthesis; Bacterial Proteins; Base Sequence; DNA, Superhelical; DNA-Binding Proteins/biosynthesis; Gene Expression Regulation; Interphase/genetics; Molecular Sequence Data; Phenotype; Transcription, Genetic
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