PMID:7689113
| Citation |
Hagervall, TG, Tuohy, TM, Atkins, JF and Björk, GR (1993) Deficiency of 1-methylguanosine in tRNA from Salmonella typhimurium induces frameshifting by quadruplet translocation. J. Mol. Biol. 232:756-65 |
|---|---|
| Abstract |
The trmD gene encodes the tRNA(m1G37)methyltransferase, which methylates guanosine (G) to 1-methylguanosine (m1G) at position 37 of tRNAs that read CUN (leucine), CCN (proline), and CGG (arginine) codons. A mutant, trmD3, has previously been isolated, which at high temperature lacks m1G in tRNA, and this deficiency was correlated with a +1 frameshifting activity. In this study, the mechanism of this trmD3-induced frameshift involving mutant tRNA(Pro) and tRNA(Leu) species has been investigated. Potential frameshifting sites for proline tRNAs, CCC-N, were efficiently suppressed in the mutant strain. Hybrid beta-galactosidases encoded by plasmid constructs containing the sites CCC-U and CCC-A were subjected to amino-terminal sequencing. The protein sequences demonstrated that a quadruplet translocation had occurred and that a proline was inserted at these sites, suggesting that a tRNA(Pro) deficient in m1G is the frameshifting agent. Therefore, a mechanism involving a quadruplet codon-anticodon interaction is favoured for trmD3-dependent +1 frameshifting. Of the four potential sites for tRNA(Leu) (CCU-N), two, CCU-U and CCU-C, were significantly suppressed in the trmD3 mutant. Thus, species of tRNA(Leu) may also act as +1 frameshift suppressors. No -1 frameshifting activity was found with the trmD3 mutant. |
| Links |
PubMed Online version:10.1006/jmbi.1993.1429 |
| Keywords |
Amino Acid Sequence; Base Sequence; Frameshift Mutation/genetics; Guanosine/analogs & derivatives; Guanosine/deficiency; Molecular Sequence Data; RNA, Bacterial/genetics; RNA, Transfer/genetics; RNA, Transfer, Leu/genetics; RNA, Transfer, Pro/genetics; Salmonella typhimurium/genetics; Suppression, Genetic/genetics; beta-Galactosidase/genetics; beta-Lactamases/genetics |
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