PMID:7683101
| Citation |
Watanabe, K and Ohta, T (1993) Mutation enhancing effect of o-vanillin in the lacZ gene of Escherichia coli: characterization of mutational spectrum. Mutat. Res. 302:13-8 |
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| Abstract |
The enhancing effect of o-vanillin (2-hydroxy-3-methoxybenz-aldehyde) on mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was characterized with mutational specificity. The mutational spectrum of MNNG-induced mutations in the presence of o-vanillin was compared with that in the absence of o-vanillin by means of a series of mutant lacZ genes in E. coli that can detect each of the six types of base substitutions and five kinds of frameshift events. In the absence of o-vanillin, the mutational spectrum induced by MNNG consisted mainly of G.C-->A.T transitions and, to a lesser extent, -1(G.C) frameshift mutations. By adding o-vanillin at 75 micrograms/plate, a marked enhancement was observed in two transitions, G.C-->A.T and A.T-->G.C, and in two frameshift mutations, +1(G.C) and -1(G.C). Only a small change was observed in the -2(C.G-G.C) fraction. Regarding the MNNG-induced frameshifts at the A.T base pair, the +1(A.T) frameshift was much more enhanced by o-vanillin than the -1(A.T) frameshift. |
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| Keywords |
Benzaldehydes/administration & dosage; Dose-Response Relationship, Drug; Drug Synergism; Escherichia coli/genetics; Frameshift Mutation/drug effects; Genes, Bacterial/drug effects; Methylnitronitrosoguanidine/administration & dosage; Mutagenicity Tests; Mutagens; Point Mutation/drug effects; beta-Galactosidase/genetics |
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