PMID:7653150

Citation	Hiszczyńska-Sawicka, E and Kur, J (1995) Binding of Escherichia coli integration host factor (IHF) to the origin segment of p15A plasmid. Acta Biochim. Pol. 42:103-8
Abstract	The integration host factor (IHF) is a sequence-specific, histone-like, multi-functional DNA-binding and -bending protein of Escherichia coli. Characterization and functional analysis of this protein has been carried out mainly in bacteriophage lambda and other mobile genetic elements. In this paper we report data concerning the binding of IHF protein to the plasmid orip15A region. IHF binds to the single site of the DNA fragment containing the orip15A, as shown by the gel mobility shift assays and footprinting experiment. On the basis of the ihf consensus sequences published, we have been able to identify one sequence of putative ihf site into the orip15A sequence with two mismatches in relation to the consensus sequence of Kur et al., 1989, Gene 81, 1-15. One ihf binding site was also found in the oriColE1 region sequence with three mismatches in relation to this consensus sequence.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Consensus Sequence; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Integration Host Factors; Molecular Sequence Data; Mutation; Plasmids/metabolism; Replication Origin/genetics; Sequence Homology, Nucleic Acid; Transformation, Bacterial
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.