PMID:7646316
| Citation |
Burkovski, A, Weil, B and Krämer, R (1995) Glutamate excretion in Escherichia coli: dependency on the relA and spoT genotype. Arch. Microbiol. 164:24-8 |
|---|---|
| Abstract |
Glutamate excretion due to amino acid starvation was investigated in "stringent" and "relaxed" strains of Escherichia coli. The observed excretion process is relA-dependent, carrier-mediated, and glutamate-specific. After induction, excretion was detected within less than 2 min and continued for more than 5 h with a rate of 7-10 nmol (mg dry weight)-1 min-1. Using carbonyl cyanide m-chlorophenylhydrazone or polymyxin B nonapeptide, together with valinomycin, it was shown that glutamate excretion is driven by the membrane potential. |
| Links | |
| Keywords |
Biological Transport; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial/genetics; Genotype; Glutamic Acid/metabolism; Ligases/genetics; Ligases/physiology; Membrane Potentials/drug effects; Polymyxin B/analogs & derivatives; Polymyxin B/pharmacology; Pyrophosphatases/genetics; Pyrophosphatases/physiology; Valinomycin/pharmacology |
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