PMID:7629058
| Citation |
Hausner, W and Thomm, M (1995) The translation product of the presumptive Thermococcus celer TATA-binding protein sequence is a transcription factor related in structure and function to Methanococcus transcription factor B. J. Biol. Chem. 270:17649-51 |
|---|---|
| Abstract |
A gene for a putative homolog of TATA-binding protein (TBP) from Thermococcus celer has been expressed in Escherichia coli, and the function of the purified recombinant protein was studied in a Methanococcus-derived cell-free transcription system. Thermococcus TBP can replace archaeal transcription factor B (aTFB) in cell-free transcription reactions. This transcriptional activation is TATA box-dependent and occurs both on tRNA(Val) and protein-encoding genes as templates indicating that Thermococcus TBP is a general transcription factor. Antibodies raised against Thermococcus TBP bind to Methanococcus aTFB and inhibit a TFB activity. These findings demonstrate that Thermococcus TBP (like eucaryal TBPs) can direct specific transcription from TATA boxes. |
| Links | |
| Keywords |
Archaea/genetics; Archaea/metabolism; Base Sequence; Cloning, Molecular; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli; Methanococcus/genetics; Methanococcus/metabolism; Molecular Sequence Data; Oligodeoxyribonucleotides; Protein Biosynthesis; TATA Box; TATA-Box Binding Protein; Transcription Factors/genetics; Transcription Factors/metabolism |
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