PMID:7603976
| Citation |
Liberek, K, Wall, D and Georgopoulos, C (1995) The DnaJ chaperone catalytically activates the DnaK chaperone to preferentially bind the sigma 32 heat shock transcriptional regulator. Proc. Natl. Acad. Sci. U.S.A. 92:6224-8 |
|---|---|
| Abstract |
In Escherichia coli the heat shock response is under the positive control of the sigma 32 transcription factor. Three of the heat shock proteins, DnaK, DnaI, and GrpE, play a central role in the negative autoregulation of this response at the transcriptional level. Recently, we have shown that the DnaK and DnaJ proteins can compete with RNA polymerase for binding to the sigma 32 transcription factor in the presence of ATP, by forming a stable DnaJ-sigma 32-DnaK protein complex. Here, we report that DnaJ protein can catalytically activate DnaK's ATPase activity. In addition, DnaJ can activate DnaK to bind to sigma 32 in an ATP-dependent reaction, forming a stable sigma 32-DnaK complex. Results obtained with two DnaJ mutants, a missense and a truncated version, suggest that the N-terminal portion of DnaJ, which is conserved in all family members, is essential for this activation reaction. The activated form of DnaK binds preferentially to sigma 32 versus the bacteriophage lambda P protein substrate. |
| Links | |
| Keywords |
Adenosine Triphosphatases/metabolism; Adenosine Triphosphate/metabolism; Bacterial Proteins/metabolism; Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins/isolation & purification; HSP70 Heat-Shock Proteins/metabolism; Heat-Shock Proteins/isolation & purification; Heat-Shock Proteins/metabolism; Homeostasis; Kinetics; Molecular Weight; Protein Binding; Sigma Factor/isolation & purification; Sigma Factor/metabolism; Transcription Factors; Transcription, Genetic |
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