PMID:7589525
| Citation |
Johnson, MS, Rowsell, EH and Taylor, BL (1995) Investigation of transphosphorylation between chemotaxis proteins and the phosphoenolpyruvate:sugar phosphotransferase system. FEBS Lett. 374:161-4 |
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| Abstract |
Transphosphorylation between the chemotaxis proteins and phosphoenolpyruvate:sugar phosphotransferase system (PTS) from Escherichia coli was investigated by incubating the CheA, CheW and CheY proteins of the chemotaxis cascade, and Enzyme I, HPr and Enzyme IImtl of the PTS with [gamma-32P]ATP or [32P]phosphoenolpyruvate in the presence and absence of cell extract. In the absence of cell extract, ATP phosphorylated CheA, but in the presence of cell extract, Enzyme I was also phosphorylated. Phosphoenolpyruvate phosphorylated only PTS components. The transphosphorylation of Enzyme I by ATP did not require chemotaxis proteins, and likely occurred through acetate kinase. Regardless of phosphorylation state, the HPr protein did not inhibit the rate of ATP-dependent phosphorylation of the CheA or the CheY protein. It is concluded that chemotaxis to PTS substrates is not mediated by transphosphorylation between the PTS and chemotaxis systems. |
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| Keywords |
Bacterial Proteins/metabolism; Chemotaxis; Escherichia coli/enzymology; Escherichia coli/physiology; Membrane Proteins/metabolism; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism; Phosphorylation; Salmonella typhimurium/enzymology; Salmonella typhimurium/physiology |
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