PMID:7585259
| Citation |
Salavati, R and Oliver, D (1995) Competition between ribosome and SecA binding promotes Escherichia coli secA translational regulation. RNA 1:745-53 |
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| Abstract |
SecA protein, the protein translocation ATPase of Escherichia coli, autogenously regulates its translation during normal protein secretion by binding to a secretion-responsive element located near the 5' end of its gene on geneX-secA mRNA. In order to characterize this autoregulation further, RNA footprinting and primerextension inhibition (toeprinting) studies were carried out with a segment of geneX-secA RNA, 30S ribosomal subunits and tRNAfMet along with purified SecA protein. The results show that ribosome and SecA-binding sites overlap, indicating that a simple competition for binding of geneX-secA mRNA presumably governs the translation initiation step. Further analysis showed that SecA protein was able to specifically dissociate a preformed 30S-tRNAfMet-geneX-secA RNA ternary complex as indicated by the disappearance of its characteristic toeprint after SecA addition. These findings are consistent with secA autoregulation, and they suggest a novel mechanism for the autoregulatory behavior of this complex protein. |
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| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Binding, Competitive; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Membrane Transport Proteins; Molecular Sequence Data; Protein Binding; Protein Biosynthesis; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; RNA, Transfer, Met/metabolism; Ribosomes/metabolism |
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