PMID:7557323
| Citation |
Forst, S, Kalve, I and Durski, W (1995) Molecular analysis of OmpR binding sequences involved in the regulation of ompF in Escherichia coli. FEMS Microbiol. Lett. 131:147-51 |
|---|---|
| Abstract |
OmpR, the transcriptional regulatory protein of ompF, had not been previously shown to specifically bind to the -70 to -60-bp region of ompF. We show that the -102 to -76-bp sequence of ompF has a high affinity binding site for OmpR and produced a single OmpR/ompF complex (complex b). Extension of this DNA fragment to include an inverted repeat sequence located between the -71 and -64-bp region resulted in the formation of a second, slower migrating complex (complex a). A -102 to -58-bp fragment containing a substitution of the -70 CG bp was able to form complex b, but not complex a. A mutant OmpR protein derived from a strain that can not repress ompF was unable to form complex a, while complex b was formed normally. Deletion of the -70 CG bp resulted in incomplete repression of OmpF. These results suggest that OmpR binds to the -71 to -64-bp region and that this sequence plays a role in the regulation of ompF in Escherichia coli. |
| Links | |
| Keywords |
Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Base Sequence; Binding Sites; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Protein Binding; Sequence Deletion |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.