PMID:7556165
| Citation |
Bogachev, AV, Murtazine, RA, Shestopalov, AI and Skulachev, VP (1995) Induction of the Escherichia coli cytochrome d by low delta mu H+ and by sodium ions. Eur. J. Biochem. 232:304-8 |
|---|---|
| Abstract |
Regulation of synthesis of cytochrome d in Escherichia coli has been studied using mutants with cytochrome-d--beta-galactosidase gene fusions. It was shown that various protonophorous uncouplers, when added to the growth medium, cause induction of the cytochrome d synthesis. The cytochrome-d-inducing activity of uncouplers correlates with their ability to inhibit such a delta mu (H+)-driven function as motility of the E. coli cells. An increase in the Na+ concentration in the growth medium from 1.5 mM to 25 mM results in induction of the cytochrome d synthesis. The cytochrome-d-inducing effect of uncouplers is much more pronounced when the Na+ concentration is high than when it is low. These data are in agreement with the assumption that cytochrome d is involved in the Na+ energetics substituting for the H+ energetics when the latter appears to be inefficient. Mutations in arcA or arcB genes (but not in fnr gene) completely prevent the increase in the cytochrome d level induced by uncouplers but are without effect on that induced by Na+. It is assumed that in the control of the cytochrome d synthesis, the Arc system is involved in the delta mu H+ sensing whereas sensing of delta mu Na+ (or of the Na+ concentration) is mediated by some other receptor system. |
| Links | |
| Keywords |
Cytochrome d Group/biosynthesis; Cytochrome d Group/genetics; Enzyme Induction; Escherichia coli/enzymology; Hydrogen/pharmacology; Ions; Mutation; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/genetics; Sodium/pharmacology; beta-Galactosidase/biosynthesis; beta-Galactosidase/genetics |
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