PMID:7543473
| Citation |
Zhong, P, Pratt, SD, Edalji, RP, Walter, KA, Holzman, TF, Shivakumar, AG and Katz, L (1995) Substrate requirements for ErmC' methyltransferase activity. J. Bacteriol. 177:4327-32 |
|---|---|
| Abstract |
ErmC' is a methyltransferase that confers resistance to the macrolide-lincosamide-streptogramin B group of antibiotics by catalyzing the methylation of 23S rRNA at a specific adenine residue (A-2085 in Bacillus subtilis; A-2058 in Escherichia coli). The gene for ErmC' was cloned and expressed to a high level in E. coli, and the protein was purified to virtual homogeneity. Studies of substrate requirements of ErmC' have shown that a 262-nucleotide RNA fragment within domain V of B. subtilis 23S rRNA can be utilized efficiently as a substrate for methylation at A-2085. Kinetic studies of the monomethylation reaction showed that the apparent Km of this 262-nucleotide RNA oligonucleotide was 26-fold greater than the value determined for full-size and domain V 23S rRNA. In addition, the Vmax for this fragment also rose sevenfold. A model of RNA-ErmC' interaction involving multiple binding sites is proposed from the kinetic data presented. |
| Links | |
| Keywords |
Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Base Sequence; Gene Expression Regulation, Bacterial; Kinetics; Methylation; Methyltransferases/genetics; Methyltransferases/metabolism; Molecular Sequence Data; Nucleic Acid Conformation; Oligodeoxyribonucleotides/metabolism; Peptidyl Transferases/genetics; Peptidyl Transferases/metabolism; RNA, Bacterial/metabolism; RNA, Ribosomal, 23S/metabolism; Substrate Specificity |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.